Isotope Labeling In Biomolecular Nmr
isotope labeling NMRbiomolecular NMRprotein NMR spectroscopyisotopic enrichment techniquesNMR structural biology
Isotope labeling is a crucial technique in biomolecular NMR spectroscopy, significantly enhancing the clarity and interpretability of data for the structural and dynamic analysis of macromolecules. By selectively incorporating specific isotopes like 13C and 15N, researchers can overcome spectral crowding, improve signal resolution, and facilitate the assignment of resonances, which is essential for determining protein structures, understanding ligand binding, and studying complex biomolecular interactions with high precision.